TRIM68 Antikörper (AA 395-444)

Produktnummer ABIN6741579

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch TRIM68 in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Rind (Kuh), Pferd, Schwein, Hund und Affe.

Kurzübersicht für TRIM68 Antikörper (AA 395-444) (ABIN6741579)

Target: TRIM68 (Tripartite Motif Containing 68 (TRIM68))

Reaktivität: Human, Maus, Rind (Kuh), Pferd, Schwein, Hund, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TRIM68 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-TRIM68 Antikörper

Bindungsspezifität: AA 395-444

Marke: IHC-plus™

Spezifität: Human TRIM68

Homologie: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Horse, Pig (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa395-444 of human TRIM68 (Q6AZZ1, NP_060543). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Dog, Bovine, Horse, Pig (100%), Galago, Bat (92%), Opossum (84%), Turkey, Chicken (80%), Rat (78%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 - 10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 50 μL sterile dH2O.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu TRIM68

Target: TRIM68 (Tripartite Motif Containing 68 (TRIM68))

Andere Bezeichnung: TRIM68

Hintergrund: Name/Gene ID: TRIM68
Family: Tripartite Motif

Synonyms: TRIM68, GC109, RNF137, Ro/SSA1 related protein, SS-56, Tripartite motif containing 68, RING finger protein 137, SS56, Tripartite motif-containing 68, SSA protein SS-56

Gen-ID: 55128

NCBI Accession: NP_060543

UniProt: Q6AZZ1

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling