NKRF Antikörper (C-Term)

Produktnummer ABIN6741576

Der Kaninchen Polyklonal Anti-NKRF-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, NKRF in Proben von Human, Maus, Ratte, Hund, Pferd, Kaninchen, Rind (Kuh), Meerschweinchen, Schwein, Affe, Fledermaus, Hamster und Huhn zu detektieren.

Kurzübersicht für NKRF Antikörper (C-Term) (ABIN6741576)

Target: NKRF (NFKB Repressing Factor (NKRF))

Reaktivität: Human, Maus, Ratte, Hund, Pferd, Kaninchen, Rind (Kuh), Meerschweinchen, Schwein, Affe, Fledermaus, Hamster, Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NKRF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NKRF Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Human NKRF

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human NKRF (O15226, NP_060014). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (100%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu NKRF

Target: NKRF (NFKB Repressing Factor (NKRF))

Andere Bezeichnung: NKRF / NRF

Hintergrund: Name/Gene ID: NKRF

Synonyms: NKRF, NFkB-repressing factor, ITBA4, Protein ITBA4, NF-kappaB repressing factor, NFKB repressing factor, Transcription factor NRF, NF-kappa-B-repressing factor, NRF

Gen-ID: 55922

NCBI Accession: NP_060014

UniProt: O15226