LDOC1 Antikörper (N-Term)

Produktnummer ABIN462213

Dieses Anti-LDOC1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von LDOC1 in IHC, WB und IHC (p). Geeignet für Human, Rind (Kuh), Hund, Meerschweinchen, Kaninchen und Schwein.

Kurzübersicht für LDOC1 Antikörper (N-Term) (ABIN462213)

Target: LDOC1 (Leucine Zipper, Down-Regulated in Cancer 1 (LDOC1))

Reaktivität: Human, Rind (Kuh), Hund, Meerschweinchen, Kaninchen, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LDOC1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-LDOC1 Antikörper

Bindungsspezifität: N-Term

Marke: IHC-plus™

Spezifität: Human LDOC1

Homologie: Percent identity by BLAST analysis: Human, Bovine, Rabbit, Pig, Guinea pig (100%) Dog (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human LDOC1 (O95751, NP_036449). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Panda, Bovine, Rabbit, Pig, Guinea pig (100%), Monkey, Bat, Horse (92%), Dog (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 50 μL sterile ddH2O.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu LDOC1

Target: LDOC1 (Leucine Zipper, Down-Regulated in Cancer 1 (LDOC1))

Andere Bezeichnung: LDOC1

Hintergrund: Name/Gene ID: LDOC1

Synonyms: LDOC1, Breast cancer, up-regulated 1, Mart7, BCUR1, Mar7, Protein LDOC1

Gen-ID: 23641

NCBI Accession: NP_036449

UniProt: O95751