SP4 Antikörper (C-Term)

Produktnummer ABIN6741568

Dieses Anti-SP4-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von SP4 in WB, IHC und IHC (p). Geeignet für Human, Maus, Ratte, Pferd, Kaninchen, Rind (Kuh), Hund, Affe, Schwein, Huhn und Xenopus laevis.

Kurzübersicht für SP4 Antikörper (C-Term) (ABIN6741568)

Target: SP4 (Sp4 Transcription Factor (SP4))

Reaktivität: Human, Maus, Ratte, Pferd, Kaninchen, Rind (Kuh), Hund, Affe, Schwein, Huhn, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SP4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SP4 Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Human SP4

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Xenopus (100%) Guinea pig (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human SP4 (Q02446, NP_003103). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Turkey, Zebra finch, Chicken, Lizard, Xenopus (100%), Guinea pig (92%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu SP4

Target: SP4 (Sp4 Transcription Factor (SP4))

Andere Bezeichnung: SP4

Hintergrund: Name/Gene ID: SP4

Synonyms: SP4, HF1B, Sp4 transcription factor, SPR-1, Transcription factor Sp4

Gen-ID: 6671

NCBI Accession: NP_003103

UniProt: Q02446