SSX4 Antikörper (AA 108-157)

Produktnummer ABIN462200

Der Kaninchen Polyklonal Anti-SSX4 Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, SSX4 in Proben von Human zu detektieren.

Kurzübersicht für SSX4 Antikörper (AA 108-157) (ABIN462200)

Target: SSX4 (Synovial Sarcoma, X Breakpoint 4 (SSX4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SSX4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SSX4 Antikörper

Bindungsspezifität: AA 108-157

Spezifität: Human SSX4

Homologie: Percent identity by BLAST analysis: Human (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa108-157 of human SSX4 (O60224, NP_005627). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Monkey (85%), Gibbon (80%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu SSX4

Target: SSX4 (Synovial Sarcoma, X Breakpoint 4 (SSX4))

Andere Bezeichnung: SSX4

Hintergrund: Name/Gene ID: SSX4

Synonyms: SSX4, CT5.4, Cancer/testis antigen 5.4, Protein SSX4, SSX4A, SSX4B

Gen-ID: 6759

NCBI Accession: NP_005627