ZNF165 Antikörper (AA 216-265)

Produktnummer ABIN6741560

Dieses Anti-ZNF165-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ZNF165 in WB, IHC und IHC (p). Geeignet für Human, Schwein und Affe.

Kurzübersicht für ZNF165 Antikörper (AA 216-265) (ABIN6741560)

Target: ZNF165 (Zinc Finger Protein 165 (ZNF165))

Reaktivität: Human, Schwein, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ZNF165 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ZNF165 Antikörper

Bindungsspezifität: AA 216-265

Marke: IHC-plus™

Spezifität: Human ZNF165

Homologie: Percent identity by BLAST analysis: Human (100%) Pig (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa216-265 of human ZNF165 (P49910, NP_003438). Percent identity by BLAST analysis: Human, Monkey (100%), Gorilla, Gibbon (92%), Pig (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu ZNF165

Target: ZNF165 (Zinc Finger Protein 165 (ZNF165))

Andere Bezeichnung: ZNF165

Hintergrund: Name/Gene ID: ZNF165
Family: Zinc Finger

Synonyms: ZNF165, Cancer/testis antigen 53, CT53, LD65, ZPF165, Zinc finger protein 165, ZSCAN7

Gen-ID: 7718

NCBI Accession: NP_003438

UniProt: P49910