TRIM35 Antikörper (AA 359-408)

Produktnummer ABIN6741557

Dieses Anti-TRIM35-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von TRIM35 in WB, IHC und IHC (p). Geeignet für Human, Maus, Ratte, Rind (Kuh), Meerschweinchen, Pferd, Kaninchen, Hund und Schwein.

Kurzübersicht für TRIM35 Antikörper (AA 359-408) (ABIN6741557)

Target: TRIM35 (Tripartite Motif Containing 35 (TRIM35))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Meerschweinchen, Pferd, Kaninchen, Hund, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TRIM35 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-TRIM35 Antikörper

Bindungsspezifität: AA 359-408

Marke: IHC-plus™

Spezifität: Human TRIM35

Homologie: Percent identity by BLAST analysis: Human, Dog, Bovine, Horse, Pig (100%) Rabbit (92%) Rat (91%) Mouse (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa359-408 of human TRIM35 (Q9UPQ4, NP_741983). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Marmoset, Bat (92%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu TRIM35

Target: TRIM35 (Tripartite Motif Containing 35 (TRIM35))

Andere Bezeichnung: TRIM35

Hintergrund: Name/Gene ID: TRIM35
Family: Tripartite Motif

Synonyms: TRIM35, HLS5, KIAA1098, MAIR, Tripartite motif containing 35, Tripartite motif-containing 35

Gen-ID: 23087

NCBI Accession: NP_741983

UniProt: Q9UPQ4