ST18 Antikörper (AA 899-948)

Produktnummer ABIN6741555

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ST18 in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Kaninchen, Rind (Kuh), Meerschweinchen, Pferd, Schwein, Fledermaus und Affe.

Kurzübersicht für ST18 Antikörper (AA 899-948) (ABIN6741555)

Target: ST18 (ZNF387) (Zinc Finger Protein 387 (ZNF387))

Reaktivität: Human, Maus, Kaninchen, Rind (Kuh), Meerschweinchen, Pferd, Schwein, Fledermaus, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ST18 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ST18 Antikörper

Bindungsspezifität: AA 899-948

Marke: IHC-plus™

Spezifität: Human ST18

Homologie: Percent identity by BLAST analysis: Human, Rabbit, Horse, Pig (100%) Rat, Dog, Chicken (92%) Mouse, Bovine, Guinea pig (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa899-948 of human ST18 (O60284, NP_055497). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Bat, Rabbit, Horse, Pig (100%), Galago, Rat, Dog, Turkey, Zebra finch, Chicken, Platypus (92%), Mouse, Bovine, Opossum, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu ST18

Target: ST18 (ZNF387) (Zinc Finger Protein 387 (ZNF387))

Andere Bezeichnung: ST18

Hintergrund: Name/Gene ID: ST18

Synonyms: ST18, KIAA0535, Zinc finger protein 387, ZC2HC10, ZNF387

Gen-ID: 9705

NCBI Accession: NP_055497

UniProt: O60284