PML Antikörper (AA 588-637)

Produktnummer ABIN6741549

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PML in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Meerschweinchen und Pferd.

Kurzübersicht für PML Antikörper (AA 588-637) (ABIN6741549)

Target: PML (Promyelocytic Leukemia (PML))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Meerschweinchen, Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PML Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PML Antikörper

Bindungsspezifität: AA 588-637

Marke: IHC-plus™

Spezifität: Human PML

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine (100%) Pig (92%) Horse, Guinea pig (84%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa588-637 of human PML (P29590). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Bovine (100%), Marmoset, Panda, Bat, Rabbit, Pig (92%), Horse, Guinea pig (84%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu PML

Target: PML (Promyelocytic Leukemia (PML))

Andere Bezeichnung: PML

Hintergrund: Name/Gene ID: PML
Family: Transcription factor

Synonyms: PML, PP8675, Promyelocytic leukemia, Protein PML, TRIM19, Promyelocytic leukemia protein, RING finger protein 71, MYL, RNF71

Gen-ID: 5371

UniProt: P29590

Pathways: p53 Signalweg, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus