PRDM5 Antikörper (AA 36-85)

Produktnummer ABIN6741548

Dieses Anti-PRDM5-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PRDM5 in WB, IHC und IHC (p). Geeignet für Human, Maus, Ratte, Rind (Kuh), Meerschweinchen, Pferd, Zebrafisch (Danio rerio), Kaninchen, Schwein und Affe.

Kurzübersicht für PRDM5 Antikörper (AA 36-85) (ABIN6741548)

Target: PRDM5 (PR Domain Containing 5 (PRDM5))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Meerschweinchen, Pferd, Zebrafisch (Danio rerio), Kaninchen, Schwein, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRDM5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PRDM5 Antikörper

Bindungsspezifität: AA 36-85

Marke: IHC-plus™

Spezifität: Human PRDM5

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Dog, Chicken (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human PRDM5 (Q9NQX1, NP_061169). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Dog, Turkey, Zebra finch, Chicken (92%), Bat (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu PRDM5

Target: PRDM5 (PR Domain Containing 5 (PRDM5))

Andere Bezeichnung: PRDM5

Hintergrund: Name/Gene ID: PRDM5

Synonyms: PRDM5, BCS2, PFM2, PR domain containing 5, PR domain-containing protein 5

Gen-ID: 11107

NCBI Accession: NP_061169

UniProt: Q9NQX1