NFIX Antikörper (AA 181-230)

Produktnummer ABIN6741545

Dieses Anti-NFIX-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von NFIX in WB, IHC und IHC (p). Geeignet für Human, Ratte, Maus, Hund, Rind (Kuh), Meerschweinchen, Pferd, Kaninchen, Fledermaus, Hamster, Affe und Schwein.

Kurzübersicht für NFIX Antikörper (AA 181-230) (ABIN6741545)

Target: NFIX (MLZE) (Nuclear Factor I/X (MLZE))

Reaktivität: Human, Ratte, Maus, Hund, Rind (Kuh), Meerschweinchen, Pferd, Kaninchen, Fledermaus, Hamster, Affe, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFIX Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NFIX Antikörper

Bindungsspezifität: AA 181-230

Marke: IHC-plus™

Spezifität: Human NFIX

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Zebrafish (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa181-230 of human NFIX (Q14938-3, NP_002492). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Stickleback (100%), Lizard, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu NFIX

Target: NFIX (MLZE) (Nuclear Factor I/X (MLZE))

Andere Bezeichnung: NF1A / NFIX

Hintergrund: Name/Gene ID: NFIX

Synonyms: NFIX, CTF, NF1A, NFI-X, NF-I/X, Nuclear factor I/X, TGGCA-binding protein, Nuclear factor 1/X, MRSHSS, NF1-X, Nuclear factor 1 X-type, SOTOS2

Gen-ID: 4784

NCBI Accession: NP_002492

UniProt: Q14938

Pathways: Skeletal Muscle Fiber Development