HMGN1 Antikörper (AA 36-85)

Produktnummer ABIN6741544

Der Kaninchen Polyklonal Anti-HMGN1-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, HMGN1 in Proben von Human, Maus, Ratte, Rind (Kuh), Pferd und Affe zu detektieren.

Kurzübersicht für HMGN1 Antikörper (AA 36-85) (ABIN6741544)

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Pferd, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HMGN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-HMGN1 Antikörper

Bindungsspezifität: AA 36-85

Marke: IHC-plus™

Spezifität: Human HMGN1

Homologie: Percent identity by BLAST analysis: Human, Bovine (100%) Rabbit (92%) Rat (84%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human HMGN1 (P05114, NP_004956). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Baboon, Monkey, Bovine, Horse, Armadillo (100%), Gibbon, Rabbit (92%), Opossum (91%), Platypus (86%), Marmoset, Pig (85%), Rat (84%), Mouse (76%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu HMGN1

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Andere Bezeichnung: HMGN1

Hintergrund: Name/Gene ID: HMGN1

Synonyms: HMGN1, HMG14

Gen-ID: 3150

NCBI Accession: NP_004956

UniProt: P05114

Pathways: Chromatin Binding