Ceramide Synthase 2 Antikörper (AA 72-121)

Produktnummer ABIN6741543

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Ceramide Synthase 2 in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Meerschweinchen, Schwein und Hamster.

Kurzübersicht für Ceramide Synthase 2 Antikörper (AA 72-121) (ABIN6741543)

Target: Ceramide Synthase 2 (CERS2)

Reaktivität: Human, Meerschweinchen, Schwein, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ceramide Synthase 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Ceramide Synthase 2 Antikörper

Bindungsspezifität: AA 72-121

Marke: IHC-plus™

Spezifität: Human LASS2

Homologie: Percent identity by BLAST analysis: Pig (100%) Mouse, Horse (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa72-121 of human CERS2 (Q96G23, NP_071358). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Hamster, Pig, Guinea pig (100%), Orangutan, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Rabbit, Horse, Opossum (92%), Bovine, Bat (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu Ceramide Synthase 2

Target: Ceramide Synthase 2 (CERS2)

Andere Bezeichnung: L3 / LASS2

Hintergrund: Name/Gene ID: CERS2

Synonyms: CERS2, Ceramide synthase 2, LAG1 longevity assurance 2, L3, LASS2, SP260, TMSG1

Gen-ID: 29956

NCBI Accession: NP_071358

UniProt: Q96G23