PAX4 Antikörper (AA 166-215)

Produktnummer ABIN462174

Dieses Anti-PAX4-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PAX4 in WB, IHC und IHC (p). Geeignet für Human.

Kurzübersicht für PAX4 Antikörper (AA 166-215) (ABIN462174)

Target: PAX4 (Paired Box 4 (PAX4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAX4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PAX4 Antikörper

Bindungsspezifität: AA 166-215

Marke: IHC-plus™

Spezifität: Human PAX4

Homologie: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Dog, Bovine, Rabbit, Pig (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa166-215 of human PAX4 (A4D0Z1, NP_006184). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Baboon, Monkey (100%), Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (92%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu PAX4

Target: PAX4 (Paired Box 4 (PAX4))

Andere Bezeichnung: PAX4

Hintergrund: Name/Gene ID: PAX4

Synonyms: PAX4, KPD, Paired box protein Pax-4, Paired domain gene 4, Paired box 4, Paired box gene 4, MODY9

Gen-ID: 5078

NCBI Accession: NP_006184

UniProt: O43316