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GATA6 Antikörper (C-Term)

Der Kaninchen Polyklonal Anti-GATA6-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, GATA6 in Proben von Human, Maus, Ratte, Rind (Kuh), Meerschweinchen, Pferd, Fledermaus und Affe zu detektieren.
Produktnummer ABIN462170

Kurzübersicht für GATA6 Antikörper (C-Term) (ABIN462170)

Target

Alle GATA6 Antikörper anzeigen
GATA6 (GATA Binding Protein 6 (GATA6))

Reaktivität

  • 59
  • 15
  • 12
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Maus, Ratte, Rind (Kuh), Meerschweinchen, Pferd, Fledermaus, Affe

Wirt

  • 56
  • 6
Kaninchen

Klonalität

  • 56
  • 6
Polyklonal

Konjugat

  • 38
  • 5
  • 4
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser GATA6 Antikörper ist unkonjugiert

Applikation

  • 38
  • 34
  • 13
  • 8
  • 6
  • 4
  • 4
  • 2
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Bindungsspezifität

    • 14
    • 9
    • 8
    • 7
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Marke

    IHC-plus™

    Spezifität

    Human GATA6

    Homologie

    Percent identity by BLAST analysis: Human, Horse (100%) Sheep, Rabbit, Pig (92%) Rat, Bovine (85%) Guinea pig (84%).

    Aufreinigung

    Immunoaffinity purified

    Immunogen

    Synthetic peptide from C-Terminus of human GATA6 (Q92908, NP_005248). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Elephant, Bat, Horse (100%), Sheep, Rabbit, Pig (92%), Rat, Panda, Bovine (85%), Guinea pig (84%), Mouse (78%).

    Type of Immunogen: Synthetic peptide

    Isotyp

    IgG
  • Applikationshinweise

    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

    Not recommended for: IF

    Kommentare

    Target Species of Antibody: Human

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Distilled water

    Konzentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handhabung

    Avoid repeat freeze-thaw cycles.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target

    GATA6 (GATA Binding Protein 6 (GATA6))

    Andere Bezeichnung

    GATA6

    Hintergrund

    Name/Gene ID: GATA6

    Synonyms: GATA6, GATA-binding factor 6, GATA-binding protein 6, GATA binding protein 6, Transcription factor GATA-6

    Gen-ID

    2627

    NCBI Accession

    NP_005248

    UniProt

    Q92908
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