EN2 Antikörper (C-Term)

Produktnummer ABIN6741528

Der Kaninchen Polyklonal Anti-EN2-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, EN2 in Proben von Human, Maus, Ratte, Meerschweinchen, Pferd, Affe und Schwein zu detektieren.

Kurzübersicht für EN2 Antikörper (C-Term) (ABIN6741528)

Target: EN2 (Engrailed Homeobox 2 (EN2))

Reaktivität: Human, Maus, Ratte, Meerschweinchen, Pferd, Affe, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EN2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-EN2 Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Human EN2

Homologie: Percent identity by BLAST analysis: Mouse, Rat, Horse, Guinea pig (100%) Xenopus (84%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human EN2 (P19622, NP_001418). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Horse, Pig, Guinea pig (100%), Bat, Opossum, Zebra finch, Chicken, Platypus (92%), Xenopus, Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.05 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu EN2

Target: EN2 (Engrailed Homeobox 2 (EN2))

Andere Bezeichnung: EN2

Hintergrund: Name/Gene ID: EN2

Synonyms: EN2, AUTS10, Engrailed homolog 2, Engrailed homeobox 2, Homeobox protein engrailed-2, Engrailed-2, Homeobox protein en-2, Hu-En-2

Gen-ID: 2020

NCBI Accession: NP_001418

UniProt: P19622