PHD1 Antikörper (AA 281-315)

Produktnummer ABIN6740396

Dieses Anti-PHD1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PHD1 in WB. Geeignet für Human, Maus, Ratte, Hund, Rind (Kuh), Meerschweinchen, Kaninchen, Pferd, Schwein, Affe und Fledermaus.

Kurzübersicht für PHD1 Antikörper (AA 281-315) (ABIN6740396)

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Meerschweinchen, Kaninchen, Pferd, Schwein, Affe, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PHD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PHD1 Antikörper

Bindungsspezifität: AA 281-315

Spezifität: Human EGLN2 / PHD1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig, Guinea pig (100%) Xenopus (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa281-315 of human EGLN2 (Q96KS0). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig (100%), Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Trout, Stickleback, Pufferfish, Zebrafish (92%), Salmon, Pike (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Not recommended for: IHC-P

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu PHD1

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Andere Bezeichnung: EGLN2 / PHD1

Hintergrund: Name/Gene ID: EGLN2

Synonyms: EGLN2, EIT6, EGL nine (C.elegans) homolog 2, Egl nine homolog 2, HPH-1, HPH-3, Estrogen-induced tag 6, PHD1, HIF-PH1, HIF-prolyl hydroxylase 1, HIFPH1

Gen-ID: 112398

UniProt: Q96KS0

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Cell RedoxHomeostasis