Splicing Factor 1 Antikörper (AA 517-566)

Produktnummer ABIN6736753

Dieses Anti-Splicing Factor 1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Splicing Factor 1 in WB, IHC und IHC (p). Geeignet für Human, Maus, Ratte, Hund, Kaninchen, Rind (Kuh), Pferd, Meerschweinchen, Affe, Schwein und Fledermaus.

Kurzübersicht für Splicing Factor 1 Antikörper (AA 517-566) (ABIN6736753)

Target: Splicing Factor 1 (SF1)

Reaktivität: Human, Maus, Ratte, Hund, Kaninchen, Rind (Kuh), Pferd, Meerschweinchen, Affe, Schwein, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Splicing Factor 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Splicing Factor 1 Antikörper

Bindungsspezifität: AA 517-566

Marke: IHC-plus™

Spezifität: Human SF1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa517-566 of human SF1 (Q15637, NP_004621). Percent identity by BLAST analysis: Human, Chimpanzee, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Shrew, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Armadillo (100%), Gibbon, Slime mold (92%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu Splicing Factor 1

Target: Splicing Factor 1 (SF1)

Andere Bezeichnung: SF1

Hintergrund: Name/Gene ID: SF1

Synonyms: SF1, D11S636, MBBP, ZCCHC25, Transcription factor ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, BBP, ZNF162, Splicing factor 1, ZFM1

Gen-ID: 7536

NCBI Accession: NP_004621

UniProt: Q15637

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location