Paired Box 3 Antikörper (AA 400-449)

Produktnummer ABIN214133

Der Kaninchen Polyklonal Anti-Paired Box 3-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, Paired Box 3 in Proben von Human, Maus, Ratte, Hund, Rind (Kuh), Pferd, Meerschweinchen, Kaninchen, Zebrafisch (Danio rerio), Fledermaus, Affe, Huhn und Xenopus laevis zu detektieren.

Kurzübersicht für Paired Box 3 Antikörper (AA 400-449) (ABIN214133)

Target: Paired Box 3 (PAX3)

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Pferd, Meerschweinchen, Kaninchen, Zebrafisch (Danio rerio), Fledermaus, Affe, Huhn, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Paired Box 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Paired Box 3 Antikörper

Bindungsspezifität: AA 400-449

Marke: IHC-plus™

Spezifität: Human PAX3

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig, Chicken, Xenopus (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa400-449 of human PAX3 (P23760, NP_852122). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus (100%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu Paired Box 3

Target: Paired Box 3 (PAX3)

Andere Bezeichnung: PAX3

Hintergrund: Name/Gene ID: PAX3

Synonyms: PAX3, CDHS, Paired domain gene 3, Paired box protein Pax-3, Paired domain gene HuP2, Waardenburg syndrome 1, WS1, HUP2, Paired box 3, Paired box homeotic gene 3, WS3

Gen-ID: 5077

NCBI Accession: NP_852122

Pathways: Sensory Perception of Sound, Tube Formation