Nucleobindin 1 Antikörper (C-Term)

Produktnummer ABIN6736741

Der Kaninchen Polyklonal Anti-Nucleobindin 1-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, Nucleobindin 1 in Proben von Human zu detektieren.

Kurzübersicht für Nucleobindin 1 Antikörper (C-Term) (ABIN6736741)

Target: Nucleobindin 1 (NUCB1)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nucleobindin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Nucleobindin 1 Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Human NUCB1

Homologie: Percent identity by BLAST analysis: Human (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human NUCB1 (Q02818, NP_006175). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan (100%), Monkey (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu Nucleobindin 1

Target: Nucleobindin 1 (NUCB1)

Andere Bezeichnung: NUCB1 / Nucleobindin

Hintergrund: Name/Gene ID: NUCB1

Synonyms: NUCB1, CALNUC, Nucleobindin-1, Nucleobindin 1, NUC, Nucleobindin

Gen-ID: 4924

NCBI Accession: NP_006175

UniProt: Q02818