NFIA Antikörper (AA 348-397)

Produktnummer ABIN6736739

Der Kaninchen Polyklonal Anti-NFIA-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, NFIA in Proben von Human, Maus, Ratte, Rind (Kuh), Pferd, Kaninchen, Meerschweinchen, Schwein, Huhn, Affe und Xenopus laevis zu detektieren.

Kurzübersicht für NFIA Antikörper (AA 348-397) (ABIN6736739)

Target: NFIA (Nuclear Factor I/A (NFIA))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Pferd, Kaninchen, Meerschweinchen, Schwein, Huhn, Affe, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFIA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NFIA Antikörper

Bindungsspezifität: AA 348-397

Marke: IHC-plus™

Spezifität: Human NFIA

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Horse, Guinea pig, Chicken (100%) Zebrafish (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa348-397 of human NFIA (Q8TA97, NP_005586). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus (100%), Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu NFIA

Target: NFIA (Nuclear Factor I/A (NFIA))

Andere Bezeichnung: NFIA / Nuclear Factor 1

Hintergrund: Name/Gene ID: NFIA

Synonyms: NFIA, CTF, NF1-A, NF-I/A, NFI-L, Nuclear Factor 1, TGGCA-binding protein, NFI-A, Nuclear factor 1 A-type, KIAA1439, Nuclear factor 1/A, Nuclear factor I/A

Gen-ID: 4774

NCBI Accession: NP_005586

UniProt: Q12857