NIFK Antikörper (AA 140-189)

Produktnummer ABIN6736736

Der Kaninchen Polyklonal anti-NIFK Antikörper wird verwendet zum Nachweis von NIFK in Proben von Human, Pferd und Schwein. Er wurde validiert für WB, IHC und IHC (p).

Kurzübersicht für NIFK Antikörper (AA 140-189) (ABIN6736736)

Target: NIFK (MKI67IP) (MKI67 FHA Domain-Interacting Nucleolar Phosphoprotein (MKI67IP))

Reaktivität: Human, Pferd, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NIFK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NIFK Antikörper

Bindungsspezifität: AA 140-189

Spezifität: Human MKI67IP

Homologie: Percent identity by BLAST analysis: Human (100%) Dog, Rabbit (92%) Horse (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa140-189 of human MKI67IP (Q9BYG3, NP_115766). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Rat, Panda, Dog, Rabbit (92%), Mouse, Zebra finch (91%), Galago, Hamster, Elephant, Bovine, Horse (85%), Bat (84%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu NIFK

Target: NIFK (MKI67IP) (MKI67 FHA Domain-Interacting Nucleolar Phosphoprotein (MKI67IP))

Andere Bezeichnung: NIFK / MKI67IP

Hintergrund: Name/Gene ID: NIFK

Synonyms: NIFK, HNIFK, MKI67IP, Nopp34

Gen-ID: 84365

NCBI Accession: NP_115766

UniProt: Q9BYG3