HSF1 Antikörper (C-Term)

Produktnummer ABIN6736730

Dieses Anti-HSF1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von HSF1 in WB, IHC und IHC (p). Geeignet für Human, Maus, Ratte, Rind (Kuh), Meerschweinchen, Hamster, Hund, Schwein und Pferd.

Kurzübersicht für HSF1 Antikörper (C-Term) (ABIN6736730)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Meerschweinchen, Hamster, Hund, Schwein, Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-HSF1 Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Human HSF1

Homologie: Percent identity by BLAST analysis: Human, Dog, Bovine, Pig (100%) Rabbit (92%) Mouse, Rat, Guinea pig (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human HSF1 (Q00613, NP_005517). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Hamster, Zebu, Dog, Bovine, Horse, Pig (100%), Marmoset, Panda, Rabbit, Platypus (92%), Mouse, Rat, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.06 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Andere Bezeichnung: HSF1

Hintergrund: Name/Gene ID: HSF1

Synonyms: HSF1, HSTF 1, Heat shock factor protein 1, HSF 1, HSTF1

Gen-ID: 3297

NCBI Accession: NP_005517

UniProt: Q00613