EOMES Antikörper (N-Term)

Produktnummer ABIN6736724

Dieses Anti-EOMES-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von EOMES in WB, IHC und IHC (p). Geeignet für Human, Rind (Kuh), Hund, Meerschweinchen, Pferd, Schwein und Affe.

Kurzübersicht für EOMES Antikörper (N-Term) (ABIN6736724)

Target: EOMES (Eomesodermin (EOMES))

Reaktivität: Human, Rind (Kuh), Hund, Meerschweinchen, Pferd, Schwein, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EOMES Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-EOMES Antikörper

Bindungsspezifität: N-Term

Marke: IHC-plus™

Spezifität: Human EOMES

Homologie: Percent identity by BLAST analysis: Human (100%) Mouse (92%) Dog, Bovine, Horse, Pig, Guinea pig (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human EOMES (O95936, NP_005433). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset (100%), Mouse (92%), Dog, Bovine, Bat, Elephant, Horse, Pig, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu EOMES

Target: EOMES (Eomesodermin (EOMES))

Andere Bezeichnung: Eomesodermin / EOMES

Hintergrund: Name/Gene ID: EOMES

Synonyms: EOMES, Eomesodermin, Eomesodermin homolog, T box, brain, 2, T-box brain protein 2, T-box brain2, T-brain-2, TBR-2, TBR2

Gen-ID: 8320

NCBI Accession: NP_005433

UniProt: O95936

Pathways: Stem Cell Maintenance