ETV4 Antikörper (AA 181-230)

Produktnummer ABIN214103

Der Kaninchen Polyklonal Anti-ETV4-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, ETV4 in Proben von Human, Maus, Ratte, Rind (Kuh) und Schwein zu detektieren.

Kurzübersicht für ETV4 Antikörper (AA 181-230) (ABIN214103)

Target: ETV4 (Ets Variant 4 (ETV4))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ETV4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ETV4 Antikörper

Bindungsspezifität: AA 181-230

Marke: IHC-plus™

Spezifität: Human ETV4 / PEA3

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Pig (100%) Rabbit, Horse (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa181-230 of human ETV4 (P43268, NP_001977). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Mouse, Rat, Elephant, Bovine, Pig (100%), Marmoset, Bat, Rabbit, Horse (92%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu ETV4

Target: ETV4 (Ets Variant 4 (ETV4))

Andere Bezeichnung: PEA3 / ETV4

Hintergrund: Name/Gene ID: ETV4

Synonyms: ETV4, ETS translocation variant 4, E1A-F, E1AF, Ets variant 4, PEA3, Protein PEA3, PEAS3

Gen-ID: 2118

NCBI Accession: NP_001977

UniProt: P43268