EEA1 Antikörper (AA 307-356)

Produktnummer ABIN6736714

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch EEA1 in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Hund, Kaninchen, Affe und Schwein.

Kurzübersicht für EEA1 Antikörper (AA 307-356) (ABIN6736714)

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Reaktivität: Human, Hund, Kaninchen, Affe, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EEA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-EEA1 Antikörper

Bindungsspezifität: AA 307-356

Marke: IHC-plus™

Spezifität: Human EEA1

Homologie: Percent identity by BLAST analysis: Human (100%) Mouse, Guinea pig (92%) Rat, Bovine, Horse (91%) Rabbit (84%) Pig (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa307-356 of human EEA1 (Q15075, NP_003557). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%), Galago, Mouse, Guinea pig, Platypus (92%), Rat, Bovine, Horse (91%), Elephant, Rabbit, Xenopus (84%), Bat, Pig (83%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.06 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile ddH2O

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu EEA1

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Andere Bezeichnung: EEA1

Hintergrund: Name/Gene ID: EEA1

Synonyms: EEA1, MST105, MSTP105, ZFYVE2, Early endosome antigen 1

Gen-ID: 8411

NCBI Accession: NP_003557

UniProt: Q15075

Pathways: Toll-Like Receptors Cascades