CTCF Antikörper (N-Term)

Produktnummer ABIN6736709

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CTCF in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Hund, Kaninchen, Pferd, Affe, Meerschweinchen, Fledermaus, Huhn und Hamster.

Kurzübersicht für CTCF Antikörper (N-Term) (ABIN6736709)

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Kaninchen, Pferd, Affe, Meerschweinchen, Fledermaus, Huhn, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CTCF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CTCF Antikörper

Bindungsspezifität: N-Term

Marke: IHC-plus™

Spezifität: Human CTCF

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig, Chicken (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human CTCF (P49711, NP_006556). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (100%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu CTCF

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Andere Bezeichnung: CTCF

Hintergrund: Name/Gene ID: CTCF
Family: Transcription factor

Synonyms: CTCF, 11-zinc finger protein, CCCTC-binding factor, Transcriptional repressor CTCF, CTCFL paralog

Gen-ID: 10664

NCBI Accession: NP_006556

UniProt: P49711