THRB Antikörper (AA 37-86)

Produktnummer ABIN6736434

Dieses Anti-THRB-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von THRB in WB, IHC und IHC (p). Geeignet für Human, Ratte, Pferd, Hund, Meerschweinchen, Kaninchen, Affe und Schwein.

Kurzübersicht für THRB Antikörper (AA 37-86) (ABIN6736434)

Target: THRB (Thyroid Hormone Receptor, beta (THRB))

Reaktivität: Human, Ratte, Pferd, Hund, Meerschweinchen, Kaninchen, Affe, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser THRB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-THRB Antikörper

Bindungsspezifität: AA 37-86

Marke: IHC-plus™

Spezifität: Human THRB

Homologie: Percent identity by BLAST analysis: Human, Horse (100%) Dog, Guinea pig (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa37-86 of human THRB (P10828, NP_000452). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Horse (100%), Galago, Elephant, Dog, Guinea pig (85%), Mouse, Rat (78%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. This antibody was tested in a simple peptide-based ELISA.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu THRB

Target: THRB (Thyroid Hormone Receptor, beta (THRB))

Andere Bezeichnung: NR1A2 / THRB

Hintergrund: Name/Gene ID: THRB
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: THRB, C-erba beta2, C-ERBA-2, ERBA-BETA, GRTH, Oncogene ERBA2, THRB2, TR-BETA, THRB1, NR1A2, Thyroid hormone receptor beta, Trbeta1, Trbeta2, C-erba beta1, C-ERBA-BETA, ERBA2, PRTH, THR1, Thyroid hormone receptor, beta, Trbeta

Gen-ID: 7068

NCBI Accession: NP_000452

UniProt: P10828

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Sensory Perception of Sound