PITX3 Antikörper (N-Term)

Produktnummer ABIN6736423

Dieses Anti-PITX3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PITX3 in WB, IHC und IHC (p). Geeignet für Human, Maus, Ratte, Hund, Pferd, Kaninchen, Schwein, Fledermaus und Affe.

Kurzübersicht für PITX3 Antikörper (N-Term) (ABIN6736423)

Target: PITX3 (Pituitary Homeobox 3 (PITX3))

Reaktivität: Human, Maus, Ratte, Hund, Pferd, Kaninchen, Schwein, Fledermaus, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PITX3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PITX3 Antikörper

Bindungsspezifität: N-Term

Marke: IHC-plus™

Spezifität: Human PITX3

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit, Pig (100%) Sheep, Bovine, Guinea pig (92%) Dog (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human PITX3 (O75364, NP_005020). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Bat, Rabbit, Horse, Pig (100%), Sheep, Elephant, Bovine, Guinea pig (92%), Opossum (90%), Dog (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu PITX3

Target: PITX3 (Pituitary Homeobox 3 (PITX3))

Andere Bezeichnung: PITX3 / PTX3

Hintergrund: Name/Gene ID: PITX3

Synonyms: PITX3, CTPP4, Homeobox protein PITX3, Paired-like homeodomain 3, Pituitary homeobox 3

Gen-ID: 5309

NCBI Accession: NP_005020

UniProt: O75364