LIM Domain Binding 1 Protein Antikörper (AA 303-352)

Produktnummer ABIN6736413

Dieses Anti-LIM Domain Binding 1 Protein-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von LIM Domain Binding 1 Protein in WB, IHC und IHC (p). Geeignet für Human, Maus, Ratte, Zebrafisch (Danio rerio), Hund, Rind (Kuh), Meerschweinchen, Pferd, Kaninchen, Huhn, Xenopus laevis, Schwein, Fledermaus, Hamster und Affe.

Kurzübersicht für LIM Domain Binding 1 Protein Antikörper (AA 303-352) (ABIN6736413)

Target: LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio), Hund, Rind (Kuh), Meerschweinchen, Pferd, Kaninchen, Huhn, Xenopus laevis, Schwein, Fledermaus, Hamster, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIM Domain Binding 1 Protein Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-LIM Domain Binding 1 Protein Antikörper

Bindungsspezifität: AA 303-352

Marke: IHC-plus™

Spezifität: Human LDB1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa303-352 of human LDB1 (Q86U70-2, NP_003884). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Xenopus, Zebrafish (100%), Platypus (92%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu LIM Domain Binding 1 Protein

Target: LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))

Andere Bezeichnung: CLIM2 / LDB1

Hintergrund: Name/Gene ID: LDB1

Synonyms: LDB1, CLIM-2, HLdb1, LIM domain-binding factor-1, LIM domain-binding protein 1, NLI, LIM domain binding 1, CLIM2, LDB-1, Nuclear LIM interactor

Gen-ID: 8861

NCBI Accession: NP_003884

UniProt: Q86U70

Pathways: Stem Cell Maintenance, Chromatin Binding