Cx40/GJA5 Antikörper (AA 86-135)

Produktnummer ABIN6736390

Dieses Anti-Cx40/GJA5-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Cx40/GJA5 in WB, IHC und IHC (p). Geeignet für Human, Hund, Rind (Kuh) und Pferd.

Kurzübersicht für Cx40/GJA5 Antikörper (AA 86-135) (ABIN6736390)

Target: Cx40/GJA5 (GJA5) (Gap Junction Protein, alpha 5, 40kDa (GJA5))

Reaktivität: Human, Hund, Rind (Kuh), Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cx40/GJA5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Cx40/GJA5 Antikörper

Bindungsspezifität: AA 86-135

Marke: IHC-plus™

Spezifität: Human GJA5 / Connexin 40

Homologie: Percent identity by BLAST analysis: Human (100%) Dog (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa86-135 of human GJA5 (P36382, NP_005257). Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon, Pig (92%), Monkey, Elephant, Panda, Dog, Bovine (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu Cx40/GJA5

Target: Cx40/GJA5 (GJA5) (Gap Junction Protein, alpha 5, 40kDa (GJA5))

Andere Bezeichnung: GJA5 / CX40 / Connexin 40

Hintergrund: Name/Gene ID: GJA5
Subfamily: Connexin
Family: Ion Channel

Synonyms: GJA5, Connexin 40, Connexin-40, Gap junction alpha-5 protein, ATFB11, CX40, Gap junction protein alpha 5

Gen-ID: 2702

NCBI Accession: NP_005257

UniProt: P36382

Pathways: Cell-Cell Junction Organization