ESR2 Antikörper (AA 82-131)

Produktnummer ABIN214750

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ESR2 in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Schwein, Rind (Kuh), Kaninchen und Fledermaus.

Kurzübersicht für ESR2 Antikörper (AA 82-131) (ABIN214750)

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Kaninchen, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ESR2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ESR2 Antikörper

Bindungsspezifität: AA 82-131

Marke: IHC-plus™

Spezifität: Human ESR2 / ER-Beta

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Pig (100%) Sheep, Dog, Guinea pig (92%) Rabbit (84%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa82-131 of human ESR2 (Q92731, NP_001428). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Mouse, Rat, Bovine, Bat, Pig (100%), Galago, Marmoset, Sheep, Panda, Dog, Guinea pig (92%), Hamster, Elephant, Cat (85%), Rabbit (84%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with sterile water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Andere Bezeichnung: ESR2 / ER Beta

Hintergrund: Name/Gene ID: ESR2
Subfamily: NR3 Steroid receptor
Family: NHR

Synonyms: ESR2, ESTRB, ER Beta, ERbeta, ER-BETA, ESR-BETA, ESRB, Estrogen receptor 2 (ER beta), NR3A2, Estrogen receptor beta, Estrogen receptor beta 4

Gen-ID: 2100

NCBI Accession: NP_001428

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling