BRD2 Antikörper (AA 524-573)

Produktnummer ABIN214728

Dieser Anti-BRD2 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von BRD2 in WB, IHC und IHC (p). Geeignet für Human, Maus, Rind (Kuh), Hund, Ratte, Pferd, Schwein, Hamster und Affe.

Kurzübersicht für BRD2 Antikörper (AA 524-573) (ABIN214728)

Target: BRD2 (Bromodomain Containing 2 (BRD2))

Reaktivität: Human, Maus, Rind (Kuh), Hund, Ratte, Pferd, Schwein, Hamster, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BRD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-BRD2 Antikörper

Bindungsspezifität: AA 524-573

Spezifität: Human BRD2

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig (100%) Xenopus (92%) Zebrafish (91%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa524-573 of human BRD2 (P25440, NP_005095). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Pig, Opossum (100%), Xenopus (92%), Zebrafish (91%), Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu BRD2

Target: BRD2 (Bromodomain Containing 2 (BRD2))

Andere Bezeichnung: BRD2 / RING3

Hintergrund: Name/Gene ID: BRD2
Subfamily: BRD
Family: Protein Kinase

Synonyms: BRD2, Bromodomain containing 2, FSRG1, KIAA9001, NaT, RING3, RNF3, O27.1.1, Bromodomain-containing 2, D6S113E, FSH

Gen-ID: 6046

NCBI Accession: NP_005095

UniProt: P25440

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion