Rekombinanter Histone 3 Antikörper (C-Term)
Kurzübersicht für Rekombinanter Histone 3 Antikörper (C-Term) (ABIN6731047)
Target
Alle Histone 3 (H3) Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- C-Term
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Marke
- AbFlex®
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Aufreinigung
- Protein A Chromatography
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Immunogen
- This antibody was raised against a peptide at the C-terminus of human Histone H3.
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Isotyp
- IgG
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Applikationshinweise
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Validated Applications:
ChIP: 4 - 5 µg per ChIP
WB: 0.01 - 0.5 µg/ml*
*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet; therefore, we recommend a High Salt & Sonication Protocol when preparing nuclear extracts. -
Kommentare
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AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H3, C-term was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in CHO cells.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Buffer
- Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- -20 °C
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- Histone 3 (H3) (Histone H3 (H3))
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Andere Bezeichnung
- Histone H3
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Molekulargewicht
- 17 kDa
Target
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