Rekombinanter PIWIL2 Antikörper (Sortase,His tag,AVI tag)

Produktnummer ABIN6731041

Der Maus Monoklonal anti-PIWIL2 Antikörper wird verwendet zum Nachweis von PIWIL2 in Proben von Maus. Er wurde validiert für WB.

Kurzübersicht für Rekombinanter PIWIL2 Antikörper (Sortase,His tag,AVI tag) (ABIN6731041)

Target: PIWIL2 (Piwi-Like 2 (PIWIL2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PIWIL2 Antikörper ist konjugiert mit Sortase,His tag,AVI tag

Applikation: Western Blotting (WB)

Produktdetails anti-PIWIL2 Antikörper (Sortase,His tag,AVI tag)

Verwendungszweck: AbFlex Mili / PiwiL2 antibody (rAb)

Marke: AbFlex®

Produktmerkmale: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.

Aufreinigung: Protein A Chromatography

Immunogen: This antibody was raised against full length mouse MILI / PIWIL2 (Miwi like / piwi-like 2) recombinant protein.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: WB: 0.2 - 2 μg/mL AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61143.

Kommentare:

This recombinant antibody has been generated using defined DNA sequences to achieve high specificity and high reproducibility. The unique advantages of this antibody are its flexible labeling and purification options as it contains a sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner. Every antibody also contains a 6xHis Tag, which can be used with nickel-based purification systems, and a Biotinylation Tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu PIWIL2

Target: PIWIL2 (Piwi-Like 2 (PIWIL2))

Andere Bezeichnung: Mili / PiwiL2

Hintergrund: AbFlex Mili / PiwiL2 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Mili / PiwiL2 is a member of the PIWI/AGO subfamily of RNA-binding proteins. Mili / PiwiL2 contains a Piwi and an AGO domain and binds a class of RNA called piwi-interacting RNA (piRNA), which are 24 to 30 nucleotides in length and are derived primarily derived from transposons and other repeated sequence elements. Mili / PiwiL2 is required for methylation and inactivation of retrotransposons. Mice deficient in Mili / PiwiL2 are sterile, as it is also required for spermatogenesis. It also positively regulates translation, and is required for germ line stem cell division and differentiation.

Molekulargewicht: 110 kDa

NCBI Accession: NP_001129193

Pathways: Stem Cell Maintenance