Rekombinanter PIM2 Antikörper (Sortase,His tag,AVI tag)

Produktnummer ABIN6731036

Der Sortase,His tag,AVI tag-konjugierte Maus Monoklonal anti-PIM2 Antikörper (ABIN6731036) detektiert spezifisch PIM2 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Rekombinanter PIM2 Antikörper (Sortase,His tag,AVI tag) (ABIN6731036)

Target: PIM2 (Proto-Oncogene Pim-2 (Serine Threonine Kinase) (PIM2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PIM2 Antikörper ist konjugiert mit Sortase,His tag,AVI tag

Applikation: Western Blotting (WB)

Produktdetails anti-PIM2 Antikörper (Sortase,His tag,AVI tag)

Verwendungszweck: AbFlex Pim2 antibody (rAb)

Marke: AbFlex®

Produktmerkmale: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.

Aufreinigung: Protein A Chromatography

Immunogen: This antibody was raised against a recombinant protein corresponding to human PIM2 (pim-2 oncogene).

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: WB: 0.5 - 2 μg/mL Note: For optimal results, we recommend the addition of 0.1 % Tween to all blocking solutions to reduce background. Individual optimization may be required. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61159.

Kommentare:

This recombinant antibody has been generated using defined DNA sequences to achieve high specificity and high reproducibility. The unique advantages of this antibody are its flexible labeling and purification options as it contains a sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner. Every antibody also contains a 6xHis Tag, which can be used with nickel-based purification systems, and a Biotinylation Tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu PIM2

Target: PIM2 (Proto-Oncogene Pim-2 (Serine Threonine Kinase) (PIM2))

Andere Bezeichnung: Pim2

Hintergrund: AbFlex Pim2 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. The antibody recognizes all three isoforms of human Pim2. PIM2 is a serine/threonine protein kinase in the CAMK family. PIM2 promotes cell survival in response to a variety of proliferative signals through activation of the I-kB kinase/NF-kB pathway. It also inhibits apoptosis through the phosphorylation of BAD. PIM2 has been implicated in the transformation of both T and B lymphocytes and is highly expressed in human leukemia and lymphomas, as well as adenocarcinomas and hepatocellular carcinomas.

Molekulargewicht: 37 kDa

NCBI Accession: NP_006866