Rekombinanter Histone H4 Antikörper (meLys20)

Produktnummer ABIN6731025

Dieser Maus Monoklonal Antikörper erkennt spezifisch Histone H4 in WB. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter Histone H4 Antikörper (meLys20) (ABIN6731025)

Target: Histone H4

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Histone H4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Histone H4 Antikörper

Bindungsspezifität: meLys20

Marke: AbFlex®

Aufreinigung: Protein A Chromatography

Immunogen: This antibody was raised against a peptide containing monomethyl-lysine 20 of human Histone H4.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:
Validated Applications:
WB: 0.5 - 2 µg/ml

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet; therefore, we recommend a High Salt & Sonication Protocol when preparing nuclear extracts. In addition, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background. Individual optimization may be required.

Kommentare:

AbFlex^® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H4K20me1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in CHO cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Details zu Histone H4

Target: Histone H4

Molekulargewicht: 12 kDa