Rekombinanter Histone H4 Antikörper (meLys20)

Produktnummer ABIN6731025

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Histone H4 in WB. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter Histone H4 Antikörper (meLys20) (ABIN6731025)

Target: Histone H4

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Histone H4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Histone H4 Antikörper

Bindungsspezifität: meLys20

Marke: AbFlex®

Aufreinigung: Protein A Chromatography

Immunogen: This antibody was raised against a peptide containing monomethyl-lysine 20 of human Histone H4.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:
Validated Applications:
WB: 0.5 - 2 µg/ml

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet; therefore, we recommend a High Salt & Sonication Protocol when preparing nuclear extracts. In addition, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background. Individual optimization may be required.

Kommentare:

AbFlex^® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H4K20me1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in CHO cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Details zu Histone H4

Target: Histone H4

Molekulargewicht: 12 kDa