Histone H3.3 Antikörper (K27M)

Produktnummer ABIN6731017

Der Kaninchen Polyklonal anti-Histone H3.3 Antikörper wird verwendet zum Nachweis von Histone H3.3 in Proben von Human. Er wurde validiert für WB.

Kurzübersicht für Histone H3.3 Antikörper (K27M) (ABIN6731017)

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H3.3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Histone H3.3 Antikörper

Bindungsspezifität: K27M

Verwendungszweck: Histone H3.3K27M antibody (pAb)

Aufreinigung: Affinity Purified

Immunogen: This antibody was generated using a Peptide containing a Methionine substitution at lysine 27 of human Histone H3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blot: 1:500 - 1:1,000 dilution

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu Histone H3.3

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Andere Bezeichnung: Histone H3

Hintergrund: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.Histone H3.1 and Histone H3.3 are the two main Histone H3 variants found in plants and animals. They are known to be important for gene regulation. Histone H3.1 and H3.3 have been shown to demonstrate unique genomic localization patterns thought to be associated with their specific functions in regulation of gene activity. Specifically, Histone H3.1 localization is found to coincide with genomic regions containing chromatin repressive marks (H3K9me3, H3K27me3 and DNA methylation), whereas Histone H3.3 primarily colocalizes with marks associated with gene activation (H3K4me3, H2BK120ub1, and RNA pol II occupancy). Deposition of the Histone H3.1 variant into the nucleosome correlates with the canonical DNA synthesis-dependent deposition pathway, whereas Histone H3.3 primarily serves as the replacement Histone H3 variant outside of S-phase, such as during gene transcription.Histone H3.3 point mutations (K27 and G34) are present in 1/3 of pediatric glioblastomas. Up to 78 % of diffuse intrinsic pontine gliomas (DIPGs) carries K27M and 36 % of non-brainstem gliomas carries either K27M or G34R/V mutations. H3.3K27M expression synergizes with p53 loss and PDGFRA activation in neural progenitor cells derived from human embryonic stem cells, resulting in neoplastic transformation.

NCBI Accession: NP_002098