His Tag Antikörper (DyLight 405)
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- Target Alle His Tag Antikörper anzeigen
- His Tag
- Reaktivität
- Bitte anfragen
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser His Tag Antikörper ist konjugiert mit DyLight 405
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Applikation
- Western Blotting (WB), Fluorescence Microscopy (FM), FLISA
- Spezifität
- His-tagged recombinant proteins or His-tagged proteins over-expressed in cells His Tag
- Aufreinigung
- 6X His Tag Antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
- Immunogen
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Immunogen: This 6X HIS antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Immunogen Type: Peptide
- Klon
- 33D10-D2
- Isotyp
- IgG1
- Labeling Ratio
- 2.4
- Top Product
- Discover our top product His Tag Primärantikörper
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- Applikationshinweise
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Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
FLISA Dilution: >1:20,000
Western Blot Dilution: 1:10,000-1:25,000
IF Microscopy Dilution: >1:5,000
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01 % (w/v) Sodium Azide - Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- RT,4 °C,-20 °C
- Informationen zur Lagerung
- Store 6X His Tag Antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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- Target
- His Tag
- Andere Bezeichnung
- 6X His Tag (His Tag Produkte)
- Substanzklasse
- Tag
- Hintergrund
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Synonyms: mouse anti-6X His Tag biotin conjugated Antibody, biotin conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody
Background: This antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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