Biotin Antikörper (DyLight 405)
Kurzübersicht für Biotin Antikörper (DyLight 405) (ABIN6720658)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
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Hersteller Produkt- Nr.
- 600-446-098
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Hersteller
- Rockland
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Verwendungszweck
- Biotin Antibody Dylight™ 405 Conjugated
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Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
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Aufreinigung
- Anti-Biotin antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
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Immunogen
- Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
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Isotyp
- IgG
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Labeling Ratio
- 3.0
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Applikationshinweise
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FLISA_Dilution: >1:20,000
ELISA_Dilution: 1:10,000 - 1:50,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: 1:10,000-1:25,000
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Kommentare
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The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store Anti-Biotin antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Haltbarkeit
- 12 months
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- Biotin
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Substanzklasse
- Chemical
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Hintergrund
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Synonyms: rabbit anti-Biotin Antibody Dylight™ 405 Conjugated, rabbit anti Biotin Antibody Dylight™ 405 Conjugated
Background: Anti-Biotin antibody conjugated to DyLight 405 is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Target
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