TRAC Antikörper (AA 1-114)

Produktnummer ABIN6719654

Der Kaninchen Polyklonal Anti-TRAC-Antikörper wurde für ELISA, WB, FACS und IHC validiert. Er ist geeignet, TRAC in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für TRAC Antikörper (AA 1-114) (ABIN6719654)

Target: TRAC (T Cell Receptor alpha Constant (TRAC))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TRAC Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Produktdetails anti-TRAC Antikörper

Bindungsspezifität: AA 1-114

Verwendungszweck: Anti-TCR alpha/TRAC Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-TCR alpha/TRAC Antibody Picoband® (ABIN6719654). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human TCR alpha recombinant protein (Position: I1-L114).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/m
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Choudhuri, K., Llodra, J., Roth, E. W., Tsai, J., Gordo, S., Wucherpfennig, K. W., Kam, L. C., Stokes, D. L., Dustin, M. L. Polarized release of T-cell-receptor-enriched microvesicles at the immunological synapse. Nature 507: 118-123, 2014. 2. Janeway, C. A., Jr., Travers, P., Walport, M., Shlomchik, M. J. Immunobiology: The Immune System in Health and Disease. (6th ed.). New York: Garland Science Publishing (pub.) 2005. Pp. 149-154, and 164-165.

Kommentare:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu TRAC

Target: TRAC (T Cell Receptor alpha Constant (TRAC))

Andere Bezeichnung: TRAC

Hintergrund:

Synonyms: T-cell receptor alpha chain C region, TRAC, TCRA

Tissue Specificity: Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels.

Background: T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor alpha and delta loci. Both the alpha and delta loci include V (variable), J (joining), and C (constant) segments and the delta locus also includes diversity (D) segments. The delta locus is situated within the alpha locus, between the alpha V and J segments. During T cell development, the delta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment, a V segment is then joined to the D-J gene. The alpha chain is synthesized by recombination joining a single V segment with a J segment. For both chains, the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Five variable segments can be used in either alpha or delta chains and are described by TRAV/DV symbols. Several V and J segments of the alpha locus are known to be incapable of encoding a protein and are considered pseudogenes.

Molekulargewicht: 45 kDa

Gen-ID: 28755

UniProt: P01848