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Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) (AA 10-459) Antikörper

Der Kaninchen Polyklonal Anti--Antikörper wurde für WB und ELISA validiert. Er ist geeignet, in Proben von Human zu detektieren.
Produktnummer ABIN6719648

Kurzübersicht für Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) (AA 10-459) Antikörper (ABIN6719648)

Target

Alle Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) Antikörper anzeigen
Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4)

Reaktivität

  • 4
  • 3
  • 3
Human

Wirt

  • 5
  • 1
Kaninchen

Klonalität

  • 6
Polyklonal

Konjugat

  • 6
Unkonjugiert

Applikation

  • 6
  • 1
  • 1
Western Blotting (WB), ELISA
  • Bindungsspezifität

    • 1
    • 1
    • 1
    AA 10-459

    Verwendungszweck

    Anti-MT-ND4 Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins.

    Produktmerkmale

    Anti-MT-ND4 Antibody Picoband® (ABIN6719648). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human MT-ND4 recombinant protein (Position: M10-S459).

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL
    ELISA, 0.1-0.5 μg/mL
    1. "Entrez Gene: MT-ND4 mitochondrially encoded NADH dehydrogenase 4". 2. Valdivieso AG, Marcucci F, Taminelli G, Guerrico AG, Alvarez S, Teiber ML, Dankert MA, Santa-Coloma TA (May 2007). "The expression of the mitochondrial gene MT-ND4 is downregulated in cystic fibrosis".Biochemical and Biophysical Research Communications. 356 (3): 805-9.

    Kommentare

    Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4)

    Andere Bezeichnung

    MT-ND4

    Hintergrund

    Synonyms: NADH-ubiquinone oxidoreductase chain 4, NADH dehydrogenase subunit 4, MT-ND4, MTND4, NADH4, ND4,

    Tissue Specificity: Expressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney.

    Background: NADH-ubiquinone oxidoreductase chain 4 is a protein that in humans is encoded by the mitochondrial gene MT-ND4. MT-ND4 is a subunit of the respiratory chain Complex I that is believed to belong to the minimal assembly of core proteins required to catalyze NADH dehydrogenation and electron transfer to ubiquinone (coenzyme Q10). Initially, NADH binds to Complex I and transfers two electrons to the isoalloxazine ring of the flavin mononucleotide (FMN) prosthetic arm to form FMNH2. The electrons are transferred through a series of iron-sulfur (Fe-S) clusters in the prosthetic arm and finally to coenzyme Q10 (CoQ), which is reduced to ubiquinol (CoQH2). The flow of electrons changes the redox state of the protein, resulting in a conformational change and pK shift of the ionizable side chain, which pumps four hydrogen ions out of the mitochondrial matrix.

    Molekulargewicht

    36 kDa

    Gen-ID

    4538

    UniProt

    P03905
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