RRBP1 Antikörper

Produktnummer ABIN6719628

Dieses Anti-RRBP1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von RRBP1 in WB, FACS, IHC, IF, ICC und IHC (fro). Geeignet für Human, Maus und Ratte.

Kurzübersicht für RRBP1 Antikörper (ABIN6719628)

Target: RRBP1 (Ribosome Binding Protein 1 (RRBP1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RRBP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-RRBP1 Antikörper

Verwendungszweck: Anti-RRBP1 Antibody Picoband®

Sequenz: EKEKKLTSDL GRAATRLQEL LKTTQEQLAR EKDTVKKLQE QLEKAED

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-RRBP1 Antibody Picoband® (ABIN6719628). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human RRBP1, which shares 87.2% and 89.4% amino acid (aa) sequence identity with mouse and rat RRBP1, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1. Basson, C. T., MacRae, C. A., Schoenberg-Fejzo, M., Morton, C. C., Spinner, N. B., Genin, A., Krug, E., Seidman, J. G., Seidman, C. E. Identification, characterization, and chromosomal localization of the human homolog (hES) of ES/130. Genomics 35: 628-631, 1996. 2. Rezaee, M., Isokawa, K., Halligan, N., Markwald, R. R., Krug, E. L. Identification of an extracellular 130 kDa protein involved in early cardiac morphogenesis. J. Biol. Chem. 268: 14404-14411, 1993. 3. Basson CT, MacRae CA, Schoenberg-Fejzo M, Morton CC, Spinner NB, Genin A, Krug E, Seidman JG, Seidman CE (Dec 1996). "Identification, characterization, and chromosomal localization of the human homolog (hES) of ES/130". Genomics. 35 (3): 628-31.

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu RRBP1

Target: RRBP1 (Ribosome Binding Protein 1 (RRBP1))

Andere Bezeichnung: RRBP1

Hintergrund:

Synonyms: Ribosome-binding protein 1, 180 kDa ribosome receptor homolog, RRp, ES/130-related protein, Ribosome receptor protein, RRBP1, KIAA1398

Tissue Specificity: Expressed in kidney.

Background: Ribosome-binding protein 1, also referred to as p180, is a protein that in humans is encoded by the RRBP1 gene. It is mapped to 20p12.1. This gene encodes a ribosome-binding protein of the endoplasmic reticulum (ER) membrane. Studies suggest that this gene plays a role in ER proliferation, secretory pathways and secretory cell differentiation, and mediation of ER-microtubule interactions. Alternative splicing has been observed and protein isoforms are characterized by regions of N-terminal decapeptide and C-terminal heptad repeats. Splicing of the tandem repeats results in variations in ribosome-binding affinity and secretory function. The full-length nature of variants which differ in repeat length has not been determined. Pseudogenes of this gene have been identified on chromosomes 3 and 7, and RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome, the result of a mutation in a nearby gene on chromosome 20p12.

Molekulargewicht: 200 kDa

Gen-ID: 6238

UniProt: Q9P2E9