SOX15 Antikörper (AA 1-233)

Produktnummer ABIN6719624

Der Kaninchen Polyklonal Anti-SOX15-Antikörper wurde für WB, ELISA, IHC, ICC, IF und FACS validiert. Er ist geeignet, SOX15 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für SOX15 Antikörper (AA 1-233) (ABIN6719624)

Target: SOX15 (SRY (Sex Determining Region Y)-Box 15 (SOX15))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SOX15 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-SOX15 Antikörper

Bindungsspezifität: AA 1-233

Verwendungszweck: Anti-SOX15 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-SOX15 Antibody Picoband® (ABIN6719624). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human SOX15 recombinant protein (Position: M1-L233).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Cremazy, F., Soullier, S., Berta, P., Jay, P. Further complexity of the human SOX gene family revealed by the combined use of highly degenerate primers and nested PCR. FEBS Lett. 438: 311-314, 1998. 2. Lee, H.-J., Goring, W., Ochs, M., Muhlfeld, C., Steding, G., Paprotta, I., Engel, W., Adham, I. M.Sox15 is required for skeletal muscle regeneration. Molec. Cell. Biol. 24: 8428-8436, 2004. 

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu SOX15

Target: SOX15 (SRY (Sex Determining Region Y)-Box 15 (SOX15))

Andere Bezeichnung: SOX15

Hintergrund:

Synonyms: Protein SOX-15, Protein SOX-12, Protein SOX-20, SOX15, SOX12, SOX20, SOX26, SOX27

Tissue Specificity: Widely expressed in fetal and adult tissues examined, highest level found in fetal spinal cord and adult brain and testis.

Background: Protein SOX-15 is a protein that in humans is encoded by the SOX15 gene. This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins.

Molekulargewicht: 29 kDa

Gen-ID: 6665

UniProt: O60248