BAT3 Antikörper

Produktnummer ABIN6719601

Dieses Anti-BAT3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von BAT3 in WB, ELISA, IHC, IF, ICC und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für BAT3 Antikörper (ABIN6719601)

Target: BAT3 (Large Proline-Rich Protein BAT3 (BAT3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BAT3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-BAT3 Antikörper

Verwendungszweck: Anti-BAT3/BAG6 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-BAT3/BAG6 Antibody Picoband® (ABIN6719601). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human BAT3/BAG6 recombinant protein (D15-A88).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Banerji, J., Sands, J., Strominger, J. L., Spies, T. A gene pair from the human major histocompatibility complex encodes large proline-rich proteins with multiple repeated motifs and a single ubiquitin-like domain. Proc. Nat. Acad. Sci. 87: 2374-2378, 1990. 2. Hessa, T., Sharma, A., Mariappan, M., Eshleman, H. D., Gutierrez, E., Hegde, R. S. Protein targeting and degradation are coupled for elimination of mislocalized proteins. Nature 475: 394-397, 2011. 3. Mariappan, M., Li, X., Stefanovic, S., Sharma, A., Mateja, A., Keenan, R. J., Hegde, R. S. A ribosome-associating factor chaperones tail-anchored membrane proteins. Nature 466: 1120-1124, 2010.

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu BAT3

Target: BAT3 (Large Proline-Rich Protein BAT3 (BAT3))

Andere Bezeichnung: BAG6

Hintergrund:

Synonyms: Large proline-rich protein BAG6, BAG family molecular chaperone regulator 6, BCL2-associated athanogene 6, BAG-6, HLA-B-associated transcript 3, Protein G3, Protein Scythe, BAG6, BAT3

Tissue Specificity: Expressed by immature dendritic cells.

Background: Large proline-rich protein BAT3 is a protein that in humans is encoded by the BAT3 gene. It is mapped to 6p21.33. This gene was first characterized as part of a cluster of genes located within the human major histocompatibility complex class III region. This gene encodes a nuclear protein that is cleaved by caspase 3 and is implicated in the control of apoptosis. In addition, the protein forms a complex with E1A binding protein p300 and is required for the acetylation of p53 in response to DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 150 kDa

Gen-ID: 7917

UniProt: P46379