Grancalcin Antikörper (AA 42-217)

Produktnummer ABIN6719568

Dieses Anti-Grancalcin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Grancalcin in WB, IHC, IF, ELISA, ICC und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für Grancalcin Antikörper (AA 42-217) (ABIN6719568)

Target: Grancalcin (GCA) (Grancalcin, EF-Hand Calcium Binding Protein (GCA))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Grancalcin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-Grancalcin Antikörper

Bindungsspezifität: AA 42-217

Verwendungszweck: Anti-Grancalcin/GCA Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Grancalcin/GCA Antibody Picoband® (ABIN6719568). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human Grancalcin/GCA recombinant protein (Position: A42-I217).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Boyhan A, Casimir CM, French JK, Teahan CG, Segal AW (Mar 1992). "Molecular cloning and characterization of grancalcin, a novel EF-hand calcium-binding protein abundant in neutrophils and monocytes". J Biol Chem. 267 (5): 2928-33. 2. Teahan CG, Totty NF, Segal AW (Oct 1992). "Isolation and characterization of grancalcin, a novel 28 kDa EF-hand calcium-binding protein from human neutrophils". Biochem J. 286. ( Pt 2): 549-54. 3. Hansen C, Tarabykina S, la Cour JM, Lollike K, Berchtold MW (Jun 2003). "The PEF family proteins sorcin and grancalcin interact in vivo and in vitro". FEBS Lett. 545 (2-3): 151-4. 4. "Entrez Gene: GCA grancalcin, EF-hand calcium binding protein".

Kommentare:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Grancalcin

Target: Grancalcin (GCA) (Grancalcin, EF-Hand Calcium Binding Protein (GCA))

Andere Bezeichnung: GCA

Hintergrund:

Synonyms: Grancalcin, GCA, GCL

Tissue Specificity: Detected in neutrophils and macrophages (at protein level). Highly expressed in bone marrow.

Background: Grancalcin is a protein that in humans is encoded by the GCA gene. This gene is mapped to 2q24.2. This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction, with magnesium alone, it partitions with the granule fraction, and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation.

Molekulargewicht: 25 kDa

Gen-ID: 25801

UniProt: P28676