LMAN1 Antikörper (AA 96-458)

Produktnummer ABIN6719563

Produktdetails anti-LMAN1 Antikörper

Target: LMAN1 (Lectin, Mannose-Binding, 1 (LMAN1))

Bindungsspezifität: AA 96-458

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LMAN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Verwendungszweck: Rabbit IgG polyclonal antibody for LMAN1 detection. Tested with WB, IHC-P, IHC-F, ICC/IF, FCM, Direct ELISA in Human,Mouse,Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for LMAN1 detection. Tested with WB, IHC-P, IHC-F, ICC/IF, FCM, Direct ELISA in Human,Mouse,Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human LMAN1 recombinant protein (Position: K96-M458).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL Immunohistochemistry(Frozen Section)|0.5-1 μg/mL Immunocytochemistry/Immunofluorescence|2 μg/mL Flow Cytometry|1-3 μg/1x106 cells Direct ELISA|0.1-0.5 μg/mL

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu LMAN1

Target: LMAN1 (Lectin, Mannose-Binding, 1 (LMAN1))

Andere Bezeichnung: LMAN1 (LMAN1 Produkte)

Synonyme: ERGIC-53 antikoerper, ERGIC53 antikoerper, F5F8D antikoerper, FMFD1 antikoerper, MCFD1 antikoerper, MR60 antikoerper, gp58 antikoerper, 2610020P13Rik antikoerper, AI326273 antikoerper, AU043785 antikoerper, C730041J05 antikoerper, P58 antikoerper, p58 antikoerper, LMAN1 antikoerper, cpx-iii antikoerper, cpxiii antikoerper, lman1 antikoerper, wu:fc54c09 antikoerper, wu:fi36e01 antikoerper, Xp58 antikoerper, lman1-a antikoerper, lectin, mannose binding 1 antikoerper, lectin, mannose-binding, 1 antikoerper, complexin 3 antikoerper, lectin, mannose binding 1 S homeolog antikoerper, LMAN1 antikoerper, Lman1 antikoerper, cplx3 antikoerper, lman1 antikoerper, lman1.S antikoerper

Hintergrund:

Synonyms: Protein ERGIC-53, ER-Golgi intermediate compartment 53 kDa protein, Gp58, Intracellular mannose-specific lectin MR60, Lectin mannose-binding 1, LMAN1, ERGIC53, F5F8D

Background: Protein ERGIC-53 also known as ER-Golgi intermediate compartment 53 kDa protein or lectin mannose-binding 1 is a protein that in humans is encoded by the LMAN1 gene. It is mapped to 18q21.32. The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH -sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency.

Gen-ID: 3998

UniProt: P49257