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HOOK3 Antikörper (AA 14-198)

Dieses Anti-HOOK3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von HOOK3 in WB, ELISA, IF, IHC, FACS und ICC. Geeignet für Human, Maus und Ratte.
Produktnummer ABIN6719547

Kurzübersicht für HOOK3 Antikörper (AA 14-198) (ABIN6719547)

Target

Alle HOOK3 Antikörper anzeigen
HOOK3 (Hook Homolog 3 (HOOK3))

Reaktivität

  • 24
  • 13
  • 5
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 20
  • 4
Kaninchen

Klonalität

  • 22
  • 2
Polyklonal

Konjugat

  • 14
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser HOOK3 Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 14-198

    Verwendungszweck

    Anti-HOOK3 Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins.

    Produktmerkmale

    Anti-HOOK3 Antibody Picoband® (ABIN6719547). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human HOOK3 recombinant protein (Position: E14-R198).

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
    Immunocytochemistry/Immunofluorescence, 2 μg/mL
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells
    ELISA, 0.1-0.5 μg/mL
    1. Urnavicius, L., Lau, C. K., Elshenawy, M. M., Morales-Rios, E., Motz, C., Yildiz, A., Carter, A. P. Cryo-EM shows how dynactin recruits two dyneins for faster movement. Nature 554: 202-206, 2018. 2. Walenta, J. H., Didier, A. J., Liu, X., Kramer, H. The Golgi-associated Hook3 protein is a member of a novel family of microtubule-binding proteins. J. Cell Biol. 152: 923-934, 2001.

    Kommentare

    Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    HOOK3 (Hook Homolog 3 (HOOK3))

    Andere Bezeichnung

    HOOK3

    Hintergrund

    Synonyms: Protein Hook homolog 3, h-hook3, Hhk3, HOOK3

    Tissue Specificity: Highly expressed in chromaffin cells of the adrenal medulla (at protein level). Detected in peripheral sympathetic ganglia (at protein level). Found in some paracrine cells in stomach and duodenum (at protein level).

    Background: Protein Hook homolog 3 is a protein that in humans is encoded by the HOOK3 gene. Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.

    Molekulargewicht

    83 kDa

    Gen-ID

    84376

    Pathways

    Stem Cell Maintenance, Maintenance of Protein Location
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