CALB1 Antikörper (AA 2-261)

Produktnummer ABIN6719541

Dieses Anti-CALB1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CALB1 in WB, IHC, ELISA, IF und FACS. Geeignet für Human, Ratte und Maus.

Kurzübersicht für CALB1 Antikörper (AA 2-261) (ABIN6719541)

Target: CALB1 (Calbindin (CALB1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CALB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-CALB1 Antikörper

Bindungsspezifität: AA 2-261

Verwendungszweck: Anti-Calbindin/CALB1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Calbindin/CALB1 Antibody Picoband® (ABIN6719541). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human Calbindin recombinant protein (Position: A2-N261).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunofluorescence, 5 μg/mL, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Parmentier M., "The human calbindins: cDNA and gene cloning.",Adv. Exp. Med. Biol. 255:233-240(1989). 2. Strauss K.I., Kuznicki J., Winsky L., Kawagoe J.I., Hammer M., Jacobowitz D.M.,"The mouse calretinin gene promoter region: structural and functional components.",Brain Res. Mol. Brain Res. 49:175-187(1997). 3. Gabrielides C., McCormack A.L., Hunt D.F., Christakos S.,"Brain calbindin-D28k and an Mr 29,000 calcium binding protein in cerebellum are different but related proteins: evidence obtained from sequence analysis by tandem mass spectrometry.",Biochemistry 30:656-662(1991).

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu CALB1

Target: CALB1 (Calbindin (CALB1))

Andere Bezeichnung: CALB1

Hintergrund:

Synonyms: Calbindin, Calbindin D28, D-28K, Vitamin D-dependent calcium-binding protein, avian-type, CALB1, CAB27

Tissue Specificity: Detected in skin. In the cornea, it is detected in the epithelial basement membrane, the epithelial cells, and at a lower level in stromal cells. Stratified squamous epithelia. Found in hemidesmosomes. Expressed in cornea, oral mucosa, esophagus, intestine, kidney collecting ducts, ureter, bladder, urethra and thymus but is absent in lung, blood vessels, skeletal muscle and nerves.

Background: Calbindin is a calcium-binding protein belonging to the troponin C superfamily. And it mapped to 8q21.3. Calretinin is expressed in central and peripheral nervous system and in many normal and pathological tissues. The rat and human calretinin exhibit 98 % sequence homology and 91 % homology to many other species. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum.

Molekulargewicht: 28 kDa

UniProt: P05937