CHM Antikörper (N-Term)

Produktnummer ABIN6719537

Der Kaninchen Polyklonal anti-CHM Antikörper wird verwendet zum Nachweis von CHM in Proben von Human. Er wurde validiert für WB, IF, ICC, IHC und FACS.

Kurzübersicht für CHM Antikörper (N-Term) (ABIN6719537)

Target: CHM (Choroideremia (Rab Escort Protein 1) (CHM))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-CHM Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Anti-CHM Antibody

Sequenz: QDQILENEEA IALSRKDKTI QHVEVFCYAS QDLHED

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-CHM Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CHM, which shares 88.9% amino acid (aa) sequence identity with both mouse and rat CHM.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL Immunocytochemistry/Immunofluorescence, 5 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu CHM

Target: CHM (Choroideremia (Rab Escort Protein 1) (CHM))

Andere Bezeichnung: CHM

Hintergrund:

Background: Rab escort protein 1 (REP1) also known as rab proteins geranylgeranyltransferase component A 1 is an enzyme that in humans is encoded by the CHM gene. It is mapped to Xq21.2. This gene encodes component A of the RAB geranylgeranyl transferase holoenzyme. In the dimeric holoenzyme, this subunit binds unprenylated Rab GTPases and then presents them to the catalytic Rab GGTase subunit for the geranylgeranyl transfer reaction. Rab GTPases need to be geranylgeranyled on either one or two cysteine residues in their C-terminus to localize to the correct intracellular membrane. Mutations in this gene are a cause of choroideremia, also known as tapetochoroidal dystrophy (TCD). This X-linked disease is characterized by progressive dystrophy of the choroid, retinal pigment epithelium and retina. Alternatively spliced transcript variants have been found for this gene.

Gene Full Name: CHM Rab escort protein

Molekulargewicht: 100 kDa

Gen-ID: 1121

UniProt: P24386

Pathways: Sensory Perception of Sound